DNA detection in suspected carriers of virus (SEMBV) by PCR (Polymerase chain Reaction)
1999
Ruangsri, J. | Supamattaya, K. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
DNA fragments of white spot virus (Systemic Ectodermal and Mesodermal Baculovirus: SEMBAV) were detected in various natural aquatic organisms by Polymerase Chain Reaction (PCR) technique. The suspected carriers (shrimps, crabs, fish, benthic organisms and planktons) were collected from sources near shrimp farms (East coast and West coast in southern of Thailand) during October 1997 to September 1998. First step amplification of the DNA extracted from samples consistently yielded 643 basepairs PCR product as expected. Second step amplification PCR product containing SEMBV DNA yielded an expected 330 basepairs. Specific DNA fragments of SEMBV were detected in 6 species of wild shrimp (krill Acetes sp. and Euphausia sp., snapping shrimp Alpleus euphrosyne, banana shrimp Penaeus merguiensis, speckled shrimp Metapenaeus monoceros and dwarf prawn Macrobrachium equideus). It was also detected in 2 species of wild crab (merder's mangrove crab Sesarma merderi and hermit crab Eupagurus bernberdus) and 3 species of fish (mud skipper Periophthalmus sp., goby fish Gnathogobius aliceae and blue panchax Aplocheilus panchax). Gastropod (Family Cerithidae), benthic polychaete and planktons (copepods, rotifers, moinas, nauplius and oscillatoria) were also positive for the SEMBV DNA fragments detection. These aquatic organisms were found to be reservoir hosts of SEMBV. Using both techniques demonstrated that the virus was found in carriers similar to SEMBV in the cultured shrimp Penaeus monodon with white spot syndrome. The prevalence of SEMBV in different carriers in relation to seasonal cycle and localities in discussed in this paper.
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