Clonal micropropagation of Patumma (Curcuma alismatifolia gagnep)
1997
Surawit Wannakrairoj (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Dept. of Horticulture. Laboratory for Plant Variety Development)
Patumma is a new ornamental crop with high demand for export. The species is now vulnerable to extinction. Cloning methodology is therefore needed to produce plants for export and to conserve the germplasm. Young inflorescence and rhizome could be used as source of the lateral bud explant. The rhizome had to be air-dried for a week before used. Pre-treatment in 52 deg C water for 5-10 minutes greatly reduced the bacterial contamination. Plantlets were multiplied on modified MS media with 0, 6.67, 13.32, 19.98 and 26.64 micro mol benzyladenine (BA) or 0.19, 0.56, 1.67 and 5.0 micro mol kinetin. The maximum multiplication rate of 4.83 folds was obtained when longitudinally divided rhizome was cultured on the medium with 13.32 mol BA. The result also showed that wild-collected and selected clones responded to the media similarly. When the media modified with 13.32, 15.54, 17.76 and 19.98 micro mol BA in combination with 15, 30 and 45 g/l sucrose were tested, the multiplication rate of plantlets were all the same.
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