Proto-oncogenes of genomic DNA in normal Thai domestic animals
1993
Achariya Sailasuta | Ted Tesprateep | Boonmee Sunyasootcharee | Susumu, Tateyama | Ryoji, Yamaguchi | Dai, Nosaka | Noriaki, Miyoshi (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Vertinary Science. Dept. of Pathology)
Proto-oncogenes homologous to the c-myc, c-ros-1, c-yes-1, and v-Ki-ras oncogenes of genomic DNAs in buffaloes, dogs, cats and ducks were investigated with Southern blot hybridization. High molecular-weight-genomic DNAs in each of the animals contained proto-oncogenes showing more than 65 percent homology of the proto-oncogenes differed in both number and approximate molecular weight: i.e. one or two bands at 1.6 to 18.0 kilobase (kb) in the c-myc probe, one band at 1.1 to 15.0 kb in the c-ros-1 probe, one to five bands at 0.3 to 18.0 kb in the c-yes-1 probe, and one to five bands at 1.6 to 18.0 kg in the v-Ki-ras probe, The degree of autoradiographic signal of each band among these animals was inconsistent for each probe. The results obtained should provide some data for molecular biological studies of tumors in domestic animals in Thailand.
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