Blood DNA analysis for Ehrlichia (Anaplasma) phagocytophila and Babesia spp. of dogs from Northern Poland
Skotarczak, B.(University of Szczecin (Poland). Dept. of Genetics)E-mail:[email protected] | Adamska, M.(University of Szczecin (Poland). Dept. of Genetics) | Supron, M.(University of Szczecin (Poland). Dept. of Genetics)
The aim of this paper was to determine if dogs are a reservoir for Ehrlichia phagocytophila and Babesia spp. and examine the possibility of coinfection. Canine blood was sampled, part of the material originated from dogs exhibiting symptoms of borreliosis. In an earlier study, the samples were screened for DNA from B. burgdorferi sensu lato. In order to screen for E. phagocytophila and Babesia spp. DNA, a PCR-based method was used with the following primers: EHR521/EHR747 for Ehrlichia and FOR1/REV1 for Babesia. In 192 samples only two contained E. phagocytophila DNA. One of these samples originated from a healthy dog, the other from an individual with symptoms of borreliosis. The examined samples were not positive for Babesia spp DNA. Coinfection was not discovered. The low level of E. phagocytophila infection may indicate that the domestic dog is not a reservoir for Ehrlichia and Babesia in Szczecin and northwestern Poland. Moreover, this area does not have populations of Rhipicephalus sanguineus or Dermacentor reticulatus - both of which are vectors of E. canis and B. canis and commonly induce ehrlichiosis and babesiosis in canines.
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