Short communication. Inhibition of Paenibacillus larvae subsp. larvae by the essential oils of two wild plants and their emulsifying agents
Fuselli, S.R. E-mail:[email protected] | Gende, L.B. | García de la Rosa, S.B. | Eguaras, M.J. | Fritz, R.
الأسبانية؛ قشتالية. La patología conocida como loque americana, que afecta a los estadios de larva y pupa de abejas (Apis mellifera L.), es causada por la bacteria Paenibacillus larvae subsp. larvae. Se evaluó el efecto antibacteriano in vitro de los aceites esenciales de manzanilla silvestre (Tagetes minuta L.) y tomillo andino (Acantholippia seriphioides A. Gray) frente a diferentes cepas de P. larvae subsp. larvae, así como también el posible efecto inhibitorio adicional del propilenglicol y la lecitina de soja, utilizados como emulsionantes de los aceites esenciales. Los resultados de la evaluación in vitro indican diferencias significativas entre los dos aceites esenciales emulsionados (P0,01). El tomillo andino presentó el mayor efecto inhibitorio con valores de concentración inhibitoria mínima y concentración bactericida mínima, entre 200-250 mg lE-1 y 300 mg lE-1 respectivamente. Se observó un efecto inhibitorio adicional producido por la utilización de propilenglicol como emulsionante y la ausencia de dicho efecto para el caso de la lecitina de soja.
اظهر المزيد [+] اقل [-]إنجليزي. In honey bees (Apis mellifera L.), American foulbrood (AFB) is caused by the infection of the larvae and pupae with the bacterium Paenibacillus larvae subsp. larvae. The antibacterial effects of the essential oils of wild camomile (Tagetes minuta L.) and Andean thyme (Acantholippia seriphioides A. Gray) against different strains of P. larvae subsp. larvae were evaluated in vitro. The possible inhibitory effects of two emulsifiers of these oils (propylene glycol and soybean lecithin) were also assessed. Significant differences in antibacterial action were seen between the oils and emulsifiers (P0.01). Andean thyme had the strongest antibacterial effect; the minimum inhibitory and minimum bactericide concentrations were 200-250 mg lE-1 and 300 mg lE-1 respectively. An additional inhibitory effect was seen when propylene glycol was used as an emulsifier; no such extra effect was seen when soybean lecithin was employed.
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