Growth of a clonal cell line of Helicoverpa zea (Lepidoptera: Noctuidae) in suspension culture and replication of its homologous baculovirus HzSNPV
2001
McIntosh, A.H. (USDA, Agricultural Research Service, Columbia (USA)) | Grasela, J.J. | Goodman, C.L. | Ignoffo, C.M.
A clonal cell line (BCIRL-HZ-AM1-11) of Helicoverpa zea was grown in stationary and suspension cultures in ExCell 401 sup(TM) medium containing 10% fetal bovine serum at 28 deg C. The cell population doubling time was 22 h in stationary culture as compared with 27 h in suspension culture. A lag time of approximately 24 h was observed during the first 24 h of the suspension culture following initiation but no lag time was observed in the stationary culture. Maximum viral titers were achieved in stationary and suspension cultures at 120 h (1.80 * 10**6 TCID50/ml) and 168 h (1.48 * l07 TCID50/ml), respectively, following inoculation with the Helicoverpa zea baculovirus (HzSNPV/Br-CL2). Infected cells harvested at 168 h from the 50 ml suspension culture produced a total number of occlusion bodies of 3 * 10**9.
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