Glutamate sensor using L-glutamate oxidase and its application for sensing GOT/GPT activity
2002
Arima, J. (Okayama Univ. (Japan). Faculty of Agriculture) | Tamura, T. | Shinohara, H. | Kusakabe, H. | Tanaka, H. | Inagaki, K.
L-Glutamate measurement and GOT/GPT assay was successful by H2O2 measurement using the L-glutamate oxidase with 4-aminoantipyrine / phenol method. But, in examination of oxygen electrode, immobilized L-glutamate oxidase at the cellulose membrane loses its affinity to the substrate. For application of L-glutamate oxidase to L-giutamate and GOT/GPT sensor, L-glutamate measurement was used for the amperometric determination with non-fixed enzyme. On examination of electron mediator, response for L-glutamate was observed with each of the compounds ferricyane, ferrocene-COOH, ferrocene-MeOH, and benzoquinone. L-Glutamate was measured by carbon printed tip electrode presenting the L-glutamate oxidase and ferricyane based on the principle of chronoamperometry. A linear calibration graph was obtained between 1mM and 3OmM. These results suggest that L-glutamate oxidase is able to utilize to L-glutamate sensor, and thatthere is a strong possibility to put this sensor to sensing for GOT/GPT activity.
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