Investigation by southern hybridization of PCR products of the capsid region of feline calicivirus (FCV)
2000
Sato, R. | Matsuda, A. | Hara, M.
To investigate the specificity of PCR products of feline calicivirus isolated in Japan, the DNA sequence of PCR products of there region between positions 1105 and 1739 of the capsid region (B-F) were determined, and 32 field isolates of FCV were confirmed by this sequence. Furthermore, by southern hybridization using PCR product of CFI/68 strain, the two methods were compared. The major band of the PCR products was electrophoresed to the position identical to that of 671 bp fragment of CFI/68 strain in 24/32 (75) isolates, a slightly larger band was detected in 7/32 (22) isolates, and a slightly smaller band was detected in one isolate (3%). Nucleotide homology of the PCR products, including the large band to known viruses was investigated and all these PCR products were located in the FCV capsid region. In contrast, none of the isolates were positive on southern hybridization. Therefore, it was shown that although the capsid region was specifically detected, this specificallycity could not be detected by southern hybridization, which is a general method of confirming specificity of PCR products.
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