The effects of jasmonic acid, methyl-jasmonate, and cucurbic acid on organogenesis in rhizome segments of Cymbidium kanran Makino
2002
Shimasaki, K. (Kochi Univ., Nankoku (Japan). Faculty of Agriculture) | Tsuneisi, J. | Fukumoto, Y.
Differentiation of rhizome into shoots occurred in Murashige and Skoog' medium containing 0.01-0.3 microM jasmonic acid(JA) , 0.01 and 0.03 microM metyl jasmonate(Me-JA) and 0.01-0.3 microM cucurbic acid(CUC), respectively. Differentiation was especially marked at 0.03 microM of JA and 0.01 microM Me-JA. Formation of protocorm-like bodies (PLB) from rhizome cultures observed at all treatments except for control treatment (growth regulator free). Higher concentrations of JA, Me-JA and CUC were effective for PLB formation. The average number of PLBs was highest at 3 microM Me-JA. The rate of PLB formation was 100 percent at I and 3 microM Me-JA, and 0.3-3 microM CUC treatments. Higher concentrations of Me-JA and CUC inhibited branching of rhizome cultures. Branching of the rhizome was completely inhibited by 0.1-3 microM Me-JA and I and 3 microM CUC.
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