Twenty-eight new microsatellite loci in chicken and their cross-species amplification in Japanese quail and helmeted guinea fowl
2003
Kayang, B.B. (Gifu Univ. (Japan). Faculty of Agriculture) | Inoue Murayama, M. | Takahashi, H. | Minezawa, M. | Tsudzuki, M. | Mizutani, M. | Ito, S.
Twenty-eight original chicken microsatellite markers were isolated and characterized to determine their utility as cross-reactive markers for comparative genetic mapping in the order Galliformes. Primer pairs were typed in 1 2 unrelated chickens and also tested on Japanese quail and helmeted guinea fowl deoxyribonucleic acid (DNA). Polymorphism was observed in 23 (82.1%) of the markers and the average number of alleles per locus was 2.9 while the mean heterozygosity was 0.19. Eleven (39.3%) of the chicken markers cross-reacted with Japanese quail DNA and 2 (7.1%) with helmeted guinea fowl DNA. The cross-reactive markers described would serve as useful resources for comparative genetic mapping in poultry species belonging to the order Galliformes.
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