PCR Method Based on the ogdH Gene for the Detection of Salmonella spp. from Chicken Meat Samples
2004
Jin, U.H | Kim, C.H (Dongguk University, Kyungju,;Cho, S.H (National Institute of Health, Seoul, Republic of Korea) | Kim, M.G (Korea Research Institute of Bioscience and Biotechnology, Daejon, Republic of Korea) | Ha, S.D | Kim, K.S (Chung-Ang University, Ansong, Republic of Korea) | Lee, K.H (Hankuk University of Foreign Studies, Gyungki Republic of Korea) | Kim, K.Y (Chungbuk National University, Cheongju, Republic of Korea) | Chung, D.H (Gyeongsang National University, Jinju, Republic of Korea) | Lee, Y.C (Dong-A University, Busan, Republic of Korea) )
In a previous paper, the ogdH gene that encodes 2-oxoglutarate dehydrogenase was isolated from Salmonella typhimurium. The catalytic N-terminal region in the enzyme was found to be very specific for the Salmonella species. Therefore, the aim of the present study was to detect S. typhimurium in food sources using primers designed for OGDH-1 and OGDH-2 which were based on the salmonella-specific region of the ogdH gene.
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