The investigation of anaerobic microflora species composition in the rumen of cattle
2004
Laugalis, J.(Lithuanian Veterinary Academy, Kaunas (Lithuania)) | Monkeviciene, I.(Lithuanian Veterinary Academy, Kaunas (Lithuania)) | Zelvyte, R.(Lithuanian Veterinary Academy, Kaunas (Lithuania)) | Sederevicius, A.(Lithuanian Veterinary Academy, Kaunas (Lithuania)) | Oberauskas, V.(Lithuanian Veterinary Academy, Kaunas (Lithuania))
The dependence of the quantitative and qualitative composition of the anaerobic microflora on different rations according to the method of N.O. van Gylswyk (1990) for the cultivation of the obligatory anaerobic microorganisms was studied. The experiments were carried out during the indoor period with 12 Lithuanian Black and White cows fed the rations of different structure. Two experimental groups of cows were formed according to the different rations. Concentrates for both groups of cows were given individually. Samples of rumen fluid were taken 3 hours after morning feeding and the following parameters were studied: total bacterial count (TBC), lactate fermenting bacterial count (LFBC), cellulolytic bacterial count (CBC) and presumptive identification of species was completed. It was determined that different rations effected quantitative composition of the anaerobic microflora in the rumen: when cows were fed maize silage the total bacterial count and the number of lactate fermenting bacteria increased while the number of cellulytic bacteria decreased. When cows were fed different rations the composition of microorganisms in the rumen changed: the number of Prevotella ruminicola in the rumen of the group I increased by 15.17% and Butyrivibrio fibrisolvens by 13.20%. When cows were given maize silage the number of other species increased by 37.01%.
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