Genetic variation in Dalbergia melanoxylon; a case of Ubena and Mkundi populations in Tanzania
1999
Hamisy, W.C.
The phenology and genetic variations in Dalbergia melanoxylon were investigated through review of the herbaria collected materials and Molecular studies using PCR methods based on RAMS markers. Among five primers (5DDB (CCA)5, (5DBH (CGA)5, (5DY(CT)5, (5YDH (TCG)5, and (5 YHY (GT)7G) tested for their capability to initiate DNA synthesis using two D. melanoxylon populations, (5YHY (GT)7G) gave the best result and were used throughout the study.A phenological trend was characterized by high levels of variations within the materials collected from different ecological locations and between the same locations in different years. Flowering trend was recorded starting from October to January followed by fruit setting from November to April and fruit maturation from June to August. In the genetic analysis, 11 molecular markers were scored ranging from 410 to 850 bp and used in the variation analysis. Within the selected markers, three banding patterns were identified; 4 markers were present throughout the genotypes from the two studied populations, one marker occurred only in one population and the remaining markers occurred throughout the populations in various proportions. The UPGMA dendrogram and the analysis of molecular variance revealed no partioning between the populations, with high molecular variance (92%) due to among the population compared to low variance 8%) due to within the populations genetic variance.Based on the results, phenology and genetic studies covering the geographic ranges of the species was recommended. Further, to improve the genetic quality of the collected seeds, a systematic sampling was advocated.
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