Lipase activity and changes in lipid content in germinating soybeanseeds
2004
Hussain, M.W. | Mahmood, A.,Government Coll. Univ., Lahore(Pakistan). Botany Dept.
Soybean (Glycine max L. var. 95-2 Faisalabad) seeds were grownunder laboratory conditions and investigated for changes in oil contentand lipase activity at different time intervals during early stages ofgermination. Total hexoses, sucrose and total soluble protein were alsostudied. Data suggested a linear decrease in oil content across 120 h.During first 48 h the decrease was 28% and by 120 h it was about 95%oil was exhausted. Lipase activity was maximum at 6.6 pH suggesting thatsoybean lipase had its optima in slightly acidic range. The enzymeactivity in non-germinating seeds was low and at 48 h germinating stagemaximum activity (4.4 units g-1) was recorded. In 96 and 120 h oldseedlings enzyme activity was 3.8 and 3.4 units g-1. Carbohydrateanalysis revealed small quantities of sucrose and hexose in resting seed.During germination both moieties registered about 90% increase across120 h. A massive increase in total soluble protein was recorded duringfirst 24 h of germination. This trend continued and by 120 h 3.6-foldincrease in protein was observed. The results of the present studysuggest that germinating soybean seeds could be a good source forcommercial preparation of lipase.
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