Introduction of somatic embryogenesis in spruce: Picea omorika, P. pungens Glauca, P. breweriana and P. abies
Hazubska, T.(Polish Academy of Sciences, Kornik (Poland). Inst. of Dendrology) | Szczygiel, K.(Forest Research Institute, Warsaw (Poland). Dept. of Genetics and Physiology of Forest Trees)
The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancic) Purk., P. pungens Glauca Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25 deg C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4 deg C during one week improved embryogenic tissue initiation in the explants of P. pungens. Seed explants of P. omorika produced embryogenic tissue on medium lacking glutamine. Only explants of P. abies and P. omorika showed embryogenic tissue formation
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