Comparison of the paraffine and frozen methods of histological sections preparation to evaluate structural elements of the muscle tissue
Sobczak, M. | Lachowicz, K. | Zochowska, J. | Zych, A. | Kotowicz, M.(Akademia Rolnicza, Szczecin (Poland). Zaklad Technologii Miesa)
The present work was aimed at following effects two methods of histological sections preparation (frozen and paraffine methods) on differences in muscles dissected out of the carcasses under the same conditions. The study involved 4 selected beef muscles (L, ST, SM and BF). Depending on technique of histological sections preparation, significant differences in muscle fibre's parameters values were recorded. The highest area, circumference and muscle fibre diameters were typical of sections prepared by a frozen method. The thickness of perimysium was identical, both in frozen and paraffine methods. Regardless of the significant changes in structural elements of muscle tissue sections preparated by a paraffine method it is possible to assess with a high precision a real muscle fibre area
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