Detection of white spot syndrome in cultured penaeid shrimps in Asia: Microscopic observation and polymerase chain reaction

Jiraporn Kasornchandra, Department of Fisheries, Bangkok . National Inst. of Coastal Aquaculture

Detection of white spot syndrome in cultured penaeid shrimps in Asia: Microscopic observation and polymerase chain reaction

1997

Jiraporn Kasornchandra, Department of Fisheries, Bangkok (Thailand). National Inst. of Coastal Aquaculture

Serious disease outbreak caused by a new virus has occured among cultured penaeid shrimps in Asian countries since 1993. Typical signs were white spots or patches on the inside surface of the shell and carapace and/or reddish coloration of the body. Histopathological changes observed among diseased shrimps collected from various countries exhibited widespread cellular degeneration and severe nuclear hypertrophy in cells of most tissues derived from ectodermal and mesodermal origin. Similar rod-shaped to elliptical virus particles surrounded by typical trilaminar envelope were found in nucleus of those infected cells with various sizes of 70-120 nm by 240-340 nm. This virus was tentatively confirmed to be a member of genus Non-Occluded Baculovirus (NOB) of the subfamily Nudibaculovirinae of Baculovirus. A portion of the sequences of specific DNA fragment of the Thai's isolate was used as a primer and compared to the other isolates collected from various countries. Results indicated that the causative agent of the white spot syndrome of cultured penaeid shrimps occurring in Asian countries were in fact closely related.

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