Development of standard method for investigation of Clostridium perfringens in food, water and beverage

Than-yalak Ninbodee; Sinee Juntarapootirat; Sompob Valtanamanee

Development of standard method for investigation of Clostridium perfringens in food, water and beverage

1995

Than-yalak Ninbodee | Sinee Juntarapootirat | Sompob Valtanamanee

Selective medium for investigation of C. perfringens in food, water and beverage was developed to substitute for FM-CW Agar due to no production of this medium in Japan. Five formulas of media: Clostridium welchii Brain Heart Infusion Agar (CWB), Clostridium welchii Agar (CWA), Loctose Egg-Yolk Agar (LEY), Liver Veal Agar (LV) and Modified Brain Heart Infusion Agar (Mod.BHI), were selected in this research. The quality and efficiency of these media was compared to FM-CW by using the reference standard culture of C. perfringens. The results showed that Mod.BHI is the most effective medium for observing the appearance of colony, the lecithinase reaction in egg yolk and Nagler reaction of organism. Therefore it was found that the Mod.BHI must be adjusted pH to 7.6 and boiled for sterilization then neomycin 400/ug/m/ was added. The developed medium and FM-CW were used to investigate C. perfringens, isolated from 35 samples of food, water and beverage and C. bifermentans isolated from 5 samples of food. The result showed that the developed medium, Mod. BHI, can be used to investigate C. perfringens in stead of FM-CW Agar.

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