Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
Petrova, I.(Gymnazium, Ricany u Prahy (Czech Republic)) | Rajmon, R.(Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Veterinarnich Disciplin) | Sedmikova, M.(Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Veterinarnich Disciplin)E-mail:[email protected] | Kuthanova, Z.(Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Veterinarnich Disciplin) | Jilek, F.(Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Veterinarnich Disciplin) | Rozinek, J.(Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Veterinarnich Disciplin)
The aim of the present study was to improve culture conditions for in vitro matured porcine oocytes by supplementing the culture medium with epidermal growth factor (EGF) or insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Neither EGF nor IGF-1 had any significant effect on the ageing of porcine oocytes. On the contrary, significant effects were demonstrated when the oocytes were cultured with both EGF and IGF-1. A combination of 10 microg EGF/mL with 25 ng IGF-1/mL was the most effective. Oocytes aged under these conditions retained very good developmental competence. We observed development to the morula (21%) or blastocyst (25%) stage in oocytes aged for 1 day with EGF and IGF-1. In conclusion, porcine oocytes matured in vitro were more resistant to the ageing when cultured in the presence of both EGF and IGF-1.
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