In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance
Kumar, S.(University of Horticulture and Forestry, Solan (India). Dept. of Biotechnology)E-mail:[email protected] | Kashyap, M. | Sharma, D.R.
Bulbscales of oriental lily hybrid Star Gazer were used as explants. Bulblets were formed on the basal portion of the excised bulbscales on MS medium supplemented with growth retardants, different sucrose concentrations and exposed to continuous light or dark. Alar, Cycocel and Paclobutrazol (1 mg/cubic dm) produced higher number of bulblets than controls. The number of bulblets, however, decreased with the increase in concentration of the growth retardants. The number of bulblets was higher at 90 than at 60 g/cubic dm sucrose and when the bulbscales were exposed to continuous light than to darkness. The growth retardants, higher sucrose concentration and continuous dark stimulated fresh mass of bulblets. The number of bulblets having roots and leaves decreased in medium with Alar, Cycocel and Paclobutrazol. A few bulblets produced roots and leaves in medium with 90 g/cubic dm sucrose and none of the regenerated bulblets produced leaves under continuous dark.
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