Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision
2006
Zhang, G.Q.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | Zhang, D.Q.(Zhejiang Academy of Agricultural Sciences, Hangzhou (China). Inst. of Crop Science) | Tang, G.X.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | He, Y.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | Zhou, W.J.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) E-mail:[email protected]
The present study evaluated the effects of chilling, partial desiccation, cotyledon excision and successive subculture of microspore-derived embryos on the development of Brassica napus plants. Out of the five media, all the genotypes tested showed the best response when the embryos were cultured on the half-strength Murashige and Skoog medium with 2.0 mg/cubic dm benzylaminopurine. A cold treatment for 3 or 5 d further increased frequencies of embryo germination (90.0 %) and plantlet development (58.46 %). Desiccation for one day also increased the embryo germination and plantlet development. Cutting the cotyledons of the embryos at late cotyledonary stage significantly increased the frequency of plantlet development. The highest rate of development was obtained from cultures of embryos sampled with size of less than 4.0 mm. Successive subculture further improved the germination and plantlet development.
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