Characterization of the Ferredoxin-Gogat gene (OsGog2 clone) expression in rice
2006
Mattana, M.(CNR, Milano (Italy). Istituto di Biologia e Biotecnologia Agraria) E-mail:[email protected] | Biazzi, E.(CNR, Milano (Italy). Istituto di Biologia e Biotecnologia Agraria) | Bertani, A.(CNR, Milano (Italy). Istituto di Biologia e Biotecnologia Agraria) | Coraggio, I.(CNR, Milano (Italy). Istituto di Biologia e Biotecnologia Agraria)
Ferredoxin-dependent glutamate synthase (Fd-Gogat) in leaf and root plastids is the last enzyme involved in the pathway of nitrate assimilation in higher plants. Arabidopsis thaliana expresses two different genes: the first, light regulated, specific of green tissues and the second expressed in other tissues. In this work, we investigated whether in our clone, OsGog2 AC Y12595, this gene is up-regulated by light or it is expressed under darkness. Fd-Gogat specific activity, protein and mRNA increased after light treatment in rice shoots. In roots, the activity and the protein content remained constant, whereas the mRNA was repressed by light treatment. The results obtained using a specific probe, situated in the 3' untranslated region of the OsGog2 cDNA, indicated that OsGog2 gene is up-regulated by light and that its expression is tissue specific. A dark expressed Fd-Gogat gene may be present in rice similarly as in Arabidopsis.
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