Intraspecific grouping of a harmful bloom-forming rephidophyte Heterosigma akashiwa based on its chloroplastic genetic signatures
2005
Akase, S. (Kagoshima Univ. (Japan)) | Yoshikawa, T. | Hayakawa, N. | Maeda, H. | Nagasaki, K. | Tomaru, Y. | Sakata, T.
Previously, five intraspecific genetic signatures were established at the flanking and inter-genic spacer (IGS) regions on the chloroplast genome of the typical harmful bloom-forming raphido-phyte Heterosigma akashiwo. In order to assess its diversity, grouping of 30 H. akashiwo strains isolated from coastal waters of western Japan was conducted by detecting the genetic signatures using the polymerase chain reaction (PCR) and cleaved amplified polymorphic sequence (CAPS) techniques. Consequently, they were divided into five categories: G1-G5. The present categorization results based on the chloroplastic genetic signatures did not show any apparent correlation with locality of the tested strains, but corresponded comparatively well to the categorization results based on sensitivity patterns to a double-stranded DNA virus HaV (H. akashiwo virus). These results may account for the reliability of the grouping technique based on chloroplastic genetic signatures and its applicability to the analysis of the propagation processes and population dynamics of H. akashiwo blooms.
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