Cysteine suppresses oxidative stress-induced myofibrillar proteolysis in chick myotubes
2004
Nakashima, K. (National Inst. of Livestock and Grassland Science, Tsukuba, Ibaraki (Japan)) | Masaki, S. | Yamazaki, M. | Abe, H.
The effects of cysteine as an antioxidant nutrient on change in protein modification and myofibrillar proteolysis in chick myotubes by induction of oxidative stress by H2O2 treatment were investigated. Myotubes were treated for 1h with H2O2 (1 mM). After this treatment, the H2O2 was removed and the cells were cultured in cysteine (0.1 and 1 mM) containing serumfree medium for 24 h. Protein carbonyl content as an index of protein modification and NT-methylhistidine release as an index of myofibrillar proteolysis were increased at 24 h after H2O2 treatment, and the increment was reduced by cysteine. Calpain, proteasome and cathepsin (B + L and D) activities were increased at 24 h after H2O2 treatment, and the increment was also reduced by cysteine. These results indicate that cysteine suppresses protein modification by oxidative stress, resulting in a decrease of protease acitivities, finally resulting in a decrease in myofibrillar proteolysis in chick myotubes.
اظهر المزيد [+] اقل [-]