The GAOLAOZHUANGREN1 gene encodes a putative glycosyltransferase that is critical for normal development and carbohydrate metabolism
2004
Shao, M. (Chinese Academy of Sciences, Shanghai (China)) | Zheng, H. | Hu, Y. | Liu, D. | Jang, J.C. | Ma, H. | Huang, H.
Glycosyltransferases are enzymes that catalyze the attachment of a sugar molecule to specific acceptor molecules. These enzymes have been shown to play important roles in a number of biological processes. Whereas a large number of putative glycosyltransferase genes have been identified by genomic sequencing, the functions of most of these genes are unknown. Here we report the characterization of an Arabidopsis mutant, designated gaolaozhuangrenl (glzl), which is allelic to parvus characterized recently. The glzl mutant exhibited a reduced plant stature, reduced size of organs in the shoot and dark-green leaves, indicating an important role of GLZI gene in normal development. The earliest GLZI expression appears at the shoot apical region of 4-d-old seedlings, which coincides with the onset of the glzl morphological phenotypes. GLZI is expressed in a tissue-specific and developmentally regulated manner, predominantly in the stem and silique, and moderately in the flower. GLZI expression is strong in the midrib of rosette and cauline leaves; however, its expression was not detectable in the midrib of the cotyledon. Further analyses revealed that carbohydrate composition and distribution were aberrant in the glzl mutant. These, together with the GLZI expression pattern, suggest a requirement for the GLZI function in normal sink?source transition during plant development.
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