Attempt at in vitro maturation of minke whale (Balaenoptera bonaerensis) oocytes using a portable CO2 incubator
2005
Iwayama, H.(Obihiro Univ. of Agriculture and Veterinary Medicine,Hokkaido (Japan)) | Ishikawa, H. | Ohsumi, S. | Fukui, Y.
The present study was conducted to investigate whether a portable CO2 incubator was effective for in vitro maturation (IVM) of bovine, porcine and minke whale oocytes, and the effect of maturation media supplemented with different hormones; porcine follicle stimulating hormone (pFSH), estradiol-17beta (E2), or pregnant mare's serum gonadotropin (PMSG): human chorionic gonadotropin (hCG) for minke whale immature oocytes was also examined. In vitro maturation rates of bovine and porcine oocytes cultured in the portable CO2 incubator were not significantly different frown the standard CO2 incubator. In minke whale IVM culture using the portable incubator, the maximum expansion of cumulus mass was ob5erved by pFSH/E2 and PMSC/hCG at the end of IVM culture. Moreover, the IVM culture period was shortened to 28-30 h from 96-120 h previously supplemented with pFSH/E2 reported. The proportion of matured oocytes cultured in the medium (26.7%) was significantly higher (P0.05) than that with PMSC/hCG (6.9%) The present 5tudy indicates that a portable CO2 incubator is a useful device for minke whale IVM culture on a research base ship, and the addition of pFSH/E2 into an IVM medium enhanced cumulus expansion and the proportion of minke whale matured oocytes.
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