Hydrolysis of docosahexaenoic acid from fish oil in lecithin based W/O microemulsion
2005
Koike, H.(Nihon Univ., Tokyo (Japan)) | Imai, M. | Suzuki, I.
Lipase catalyzed hydrolysis reaction of fatty acid bonding with triglyceride docosahexaenoic acid (DHA) was attained with the aid of soybean lecithin based W/O microemulsion. This reaction was fruitful as a pretreatment of enrichment of DHA in triglyceride from marine bioresources. Ester-bonded DHA was hardly hydrolyzed by Candida rugosa lipases due to their steric hindrance. On the other hands, mono unsaturated fatty acid in triglyceride was easily hydrolyzed by lipase specificity. Phosphate buffer (6.0 - pH - 7.5) was used as an aqueous phase and 2,2,4-trimethylpentane was employed as an organic solvent. Molar ratio of water to lecithin presented by W0 was used as a measure of water content. Initial reaction rate was strongly depended on the Wo value and appeared a maximum at ca. Wo
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