Detection of strawberry mild yellow edge-associated potexvirus (SMYEAV) in strawberry [Fragaria ananassa] by reverse transcription and polymerase chain reaction (RT-PCR) and virus isolate in Chiba prefecture [Japan]
2005
Suzuki, T.(Chiba-ken. Government Office (Japan)) | Iijima, N. | Hanada, K. | Yoshii, S.
To establish the detection method of SMYEAV by the RT-PCR, a simple extraction method of RNA with high purity was investigated. In addition, detection methods of SMYEAV by RT-PCR and by bioassay were compared. 1. The RNA extracted from the SMYEAV-infected leaves of strawberry using the modified CF-11 method included a low content of PCR inhibitory substance and the detection of SMYEAV by RTPCR was possible. 2. Compared to bioassay, RT-PCR was quicker to detect and higher in detection sensitivity. 3. The base sequence of SMYEAV emerged in Chiba prefecture was 82.7-84.2% homologous to that of SMYEAV isolate, MY-18, and 83.8-89.2% homologous to SMYEAV isolate, D-74.
اظهر المزيد [+] اقل [-]