Structural characterization of long unit-chains of amylopectin
2005
Hanashiro, I.(Kagoshima Univ. (Japan). Faculty of Agriculture) | Matsugasako, J. | Egashira, T. | Takeda, Y.
Long chains (LC) were fractionated by 1-butanol precipitation from isoamylase-debranched amylopectins of rice, maize, wheat, buckwheat, and sweet potato. The structure of the precipitate (LCppt) was characterized by high-performance size-exclusion chromatography (HPSEC) with pre-column labeling with 2-aminopyridine. HPSEC showed that LCppt corresponded to the LC fraction of unit-chain distribution of amylopectin. LCppt had a number-average degree of polymerization of 330-490 and an average-number of chains of 1.2-1.4, indicating that the structure of LCppt is very similar irrespective of its botanical sources and is distinct from amylose. HPSEC revealed that size distribution of LCppt was also similar among the specimens examined. The distribution was distinct from and much narrower than that of amylose. Still, LCppt consisted of a significant amount (by weight) of chains as long as amylase. LCppt contained 5.1-9.4 mol% of branched molecules, which had -5 chains per molecule (except for 10 of indica rice IR36), indicating incomplete debranching of the side chains on LC by isoamylase. The structure of the isoamylase-resistant branches is unknown. In this respect, LCppt and amylose appear to have a common branched structure, which is unusual in starch alpha-glucans.
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