Characterization of ACE-inhibitory peptide from hydrolysis product of egg yolk protein and its hypotensive effect in SHR rat
2006
Kanada, T.(Bizen Chemical Co. Ltd., Akaiwa, Okayama (Japan)) | Ishikawa, H. | Kosugi, K. | Akiyama, J. | Hada, T. | Kawasaki, H.
We obtained ACE inhibitory hydrolysis products from egg yolk protein by enzyme digestion. The egg yolk protein was digested with four types of proteases (Bioprase-SP-15FG, Denapsin, Denazyme AP, and papain; Nagase ChemteX). These hydrolysis products were assayed for their effect on ACE inhibitory activity in vitro. The hydrolysis product obtained by digestion with Bioprase-SP-15FG showed the most potent inhibitory activity (IC50=33.2 mug/mL) and had a strong hypotensive effect when administered orally to spontaneously hypertensive rats (1,000 mg/kg). One of the active products against ACE activity was purified by reverse-phase HPLC and identified as Ile-Tyr with an IC50 Value of 2.4 muM (0.71 mug/mL).
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