Molecular Characterization of Epoxide Hydrolase from Aspergillus niger LK using Phylogenetic Analysis
Lee, E.Y.;Lee, S.J.;Lee, J.W.;Kim, H.S.(Kyungsung University, Pusan, Republic of Korea)E-mail:[email protected]
A gene coding for epoxide hydrolase (EH) of Aspergillus niger LK, a fungus possessing the enantioselective hydrolysis activity for racemic epoxides, was characterized by phylogenetic analysis. The deduced protein of A. niger LK epoxide hydrolase shares significant sequence similarity with several bacterial EHs and mammalian microsomal EHs (mEH) and belongs to the a/β hydrolase fold family. EH from A. niger LK had 90.6% identity with 3D crystal structure of lqo7 in Protein Data Bank. Sequence comparison with other source EHs suggested that Asp∨l92, Asp∨348 and His∨374 constituted the catalytic triad.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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