Enhancement of β-D-Glucans Production by Agaricus blazei Murill by Nitrogen Supplementation
2005
Na, J.G. (STR Biotech Co., Ltd., Hi-Tech Venture Town, Chuncheon, Republic of Korea) | Kim, H.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) | Chun, G.T. (Kangwon National University, Chuncheon, Republic of Korea) | Chang, Y.K. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: [email protected] | Lee, S.J. (STR Biotech Co., Ltd., Hi-Tech Venture Town, Chuncheon, Republic of Korea) | Chung, Y.H. (Kangwon National University, Chuncheon, Republic of Korea)
Temporal changes of cell growth pattern and intracellular content of β-D-glucans were investigated with off-gas data in Agaricus blazei culture where glucose was intermittently fed. It was observed that the time point of carbon source depletion coincided with the point of sudden drop in the carbon dioxide evolution rate (CER), and that the sole supplementation of glucose was not enough to maintain active cell growth and glucan content. On the other hand, when yeast extract, a typical nitrogen source, was supplemented together with glucose when the CER suddenly dropped because of carbon source depletion, an active cell growth could be maintained until the end of the culture and the glucan content did not decrease with culture time, significantly enhancing glucan productivity.
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