Expressed sequence tags analysis of Blattella germanica
2005
Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea) | Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea) | Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea) | Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea) | Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea) | Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea) | Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: [email protected] | Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function.
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