Acclimatization of in vitro Gymnaster koraiensis Using Paclobutrazol and Transplanting Medium
2005
Kim, S.M. (Seoul National University, Seoul, Republic of Korea) | Lee, C.H. (Seoul National University, Seoul, Republic of Korea) | Kim, K.S. (Seoul National University, Seoul, Republic of Korea)
An efficient acclimatization of micropropagated Gymnaster koraiensis was developed through paclobutrazol pretreatment in vitro. Microshoots were induced on the MS medium containing a combination of 0.2 mgㆍL-¹ thidiazuron and 0.1 mgㆍL-¹ α-naphthaleneacetic acid, followed by culture on MS media containing combinations of 0.0-2.0 mgㆍL-¹ paclobutrazol (PBZ) and 0.0-0.1 mgㆍL-¹ indole-3-butyric acid (IBA) for 4 weeks. PBZ pretreatments in vitro at 0.5-1.0 mgㆍL-¹ seemed to be more effective on the number of primary roots and fresh weight of root regardless of IBA concentration. Thus, PBZ pretreatment in vitro at 0.0, 0.5, 1.0, or 2.0 mgㆍL-¹, respectively, was evaluated for in vitro root development and for acclimatization of plantlets in a greenhouse.
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