Expression of Sara2 Human Gene in Erythroid Progenitors
2005
Jardim, Denis Leonardo Fontes (State University of Campinas, Campinas, SP, Brazil) | Da Cunha, Anderson Ferreira (State University of Campinas, Campinas, SP, Brazil) | Da Silva Santos Duarte, Adriana (State University of Campinas, Campinas, SP, Brazil) | Dos Santos, Camila Oresco (State University of Campinas, Campinas, SP, Brazil) | Olalla Saad, Sara Terezinha (State University of Campinas, Campinas, SP, Brazil) | Ferreira Costa, Fernando (State University of Campinas, Campinas, SP, Brazil), E-mail: [email protected]
A human homologue of Sar1, named Sara2, was shown to be preferentially expressed during erythropoiesis in a culture stimulated by EPO. Previous studies, in yeast, have shown that secretion-associated and Ras-related protein (Sar1p) plays an essential role in protein transport from the endoplasmic reticulum to the Golgi apparatus. Here, we report the molecular analysis of Sara2 in erythroid cell culture. A 1250 bp long cDNA, encoding a 198 amino-acid protein very similar to Sar1 proteins from other organisms, was obtained. Furthermore, we also report a functional study of Sara2 with Real-time quantitative PCR analysis, demonstrating that expression of Sara2 mRNA increases during the initial stages of erythroid differentiation with EPO and that a two-fold increase in expression occurs following the addition of hydroxyurea (HU).
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