Calli culture of oil flax: establishing and regeneration
2005
Grauda, D., University of Latvia, Salaspils (Latvia). Inst. of Biology | Rashal, I., University of Latvia, Salaspils (Latvia). Inst. of Biology
By using somaclonal variation it is possible to obtain plant breeding source material with modified agronomical traits. In flax is known somaclonal variation in resistance to biotic and abiotic stress, plant height, number of seeds in a vessel and number of seeds. The best for obtaining somaclonal variation is calli culture. This study was carried out to determine the best method of obtaining oil flax calli culture. Different parts of plants were used as explants (stem segments, leaves, roots' segments). For calli culture establishment, MS basal medium with 2 mg lE-1 2.4-D was used. For inducing shoot formation, MS medium supplemented with 0.2 mg lE-1 IAA or 1 mg LE-1 BAP and MS medium without phytohormones were used. As a carbohydrates source, 30 g lE-1 of sucrose was used in all mediums. For shoots rooting, MS medium with 0.1 mg lE-1 IAA and 1 g lE-1 AgNO3 and 10 g lE-1 sucrose were used. Development of root-like structures and regeneration zones was dependent on both the used medium and explants type, which calli were derived from, but did not relate with calli size. Shoots were only observed on calli derived from leaves and grown on media with phytohormones: shoots given 5% of calli grown on MS basal medium with IAA and 72% of calli grown on MS basal medium with BAP. In total, 3-22 shots were obtained from a single callus.
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