Survey on the microorganisms present on leaf surfaces of Duranta spp. in Kiwalan, Iligan City [Philippines]
2005
Aba, B.L.L. | Lucena, M.A. | Lahoylahoy, L.D.
The study was conducted to determine the presence of microorganisms on leaf surfaces of Duranta spp. plant that is abundantly found in the viccinities of the adjacent flour and cement industries in Kiwalan, where Duranta spp. leaf surfaces were frequently exposed to these industrial debris and exhausts. A moistened cotton swab was used on the leaf surfaces of Duranta spp. which was directly swabbed into nutrient agar (NA) plates. Three leaves on each of the six plants of Duranta spp. in this area were selected for the collection of samples. After 48 hours of incubation, colonies were counted and characterized. PFC site 2 (Duranta spp. plant 2) yielded the highest colony count (176.3 CFU/ml) attributed to lesser exhaust exposure due to its location. PFC site 1 (Duranta spp. plant 1) has 129.3 CFU/ml which is the lowest that accounted to much exhaust exposure. Seven isolates were incubated for 36 h and gram stained. Implications on the effects of flour and cement exhaust on leaf surfaces are important for microbial-leaf intearction studies.
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