Systemic ectodermal and mesodermal baculovirus (SEMBV) induces apoptosis in experimentally infected Penaeus monodon
1999
Worawidh Wajjwalku | Thinnawat Srisook | Orawan Boodee | Preeda Lertwatcharasarakul | Visanu Boonyawiwat | Theerapol Sirinarumitr(Kasetsart Univ., Bangkok (Thailand). Faculty of Veterinary Medicine. Dept. of Pathology)
Sytemic ectodermal and mesodernal baculovirus (SEMBV) is an economically important virus for te shrimp culure in Thailand and many countries in Asia. SMBV has been extensively studied, however, the mechanism of SEMBV-induced cell death is still unclear. It is well known that baculoviruses possess two anti-apoptotic genes (p35 and IAP). In this study, we investigated whether SEMBV kills cells by apoptosis. Serial sections of infected shrimps were subjected to in situ hybridization for the detection of viral infected cells and terminal deoxytransferase digoxigenin-dUTP nick end labeling (TUNEL) for the detection of apoptotic cells. By in situ hybridization, the virus-infected cells were sub-cuticular epithelium cells, myocardium, gill epithelial cells, hepatopancreas myoepithelial cells, skeletal muscle cells, and lymphoid cells. By TUNEL, the apoptotic cells were the same cell types of the viral infected cells, however, the number of the apoptotic cells were higher than the number of virus infected cells. At this time, we do no known whether apoptosis is induced by the defense mechanism of the shrimp or the viral products.
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