Sequence analysis of α - amylase inhibitors genes from Triticum dicoccoides
2006
Jiang Bin | Wang Jirui | Wei Yumin
صينى. 对3份野生二粒小麦（Triticum dicoccoides）材料的抗虫24kDa α-淀粉酶抑制因子编码基因进行分离克隆，得到72个24kDa α-淀粉酶抑制因子基因，并进行序列分析。结果发现，α-淀粉酶抑制因子在野生二粒小麦中以多拷贝形式存在，经卡方检验初步推断野生二粒小麦中的α-淀粉酶抑制因子成熟蛋白编码基因序列变异类型在供试材料间无显著差异。序列分析表明，24kDa α-淀粉酶抑制因子成熟蛋白编码基因序列无论在野生二粒小麦种内还是与小麦属的其他物种之间，都具有很高的一致性，说明该基因在进化过程中十分保守。
اظهر المزيد [+] اقل [-]إنجليزي. Seventy-two genes encoding the 24kDa α- amylase inhibitors from three genotypes of T. dicoccoides were characterized. According to sequences alignment, it is found that the 24kDa α- amylase inhibitors in T. dicoccoides are encoded by multi-gene. The distribution of inhibitor forms has no distinct difference among the three accessions of T. dicoccoides by X2 test. The nucleotide sequences of 24 kDa α- amylase inhibitors are with high coherence between T. dicoccoides and related species, which indicates that the α- amylase inhibitors in this family are conserved in evolution.
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