Transformation using Conjugal Transfer and attB Site Properties of Streptomyces natalensis ATCC27448
2005
Lee, K.M. (Kyungnam University, Masan, Republic of Korea) | Choi, S.U. (Kyungnam University, Masan, Republic of Korea) | Park, H.R. (Kyungnam University, Masan, Republic of Korea) | Hwang, Y.I. (Kyungnam University, Masan, Republic of Korea), E-mail: [email protected]
Streptomyces natalensis ATCC27448 produces natamycin, a commercially important macrolide antifungal antibiotic. For molecular genetic study of S. natalensis, we have developed a system for introducing DNA into S. natalensis via conjugal transfer from Escherichia coli. An effective transformation procedure for S. natalensis was established based on transconjugation from E. coli ET12567/pUZ8002 using a ΦC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 10 mM MgCl₂ using 6.25×10∨8 of E.coli donor cells without heat treatment of spores.
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