Screening of Active Substance FS11052 as an Inhibitor of Neurotransmitter Release From PC12 Cells
2006
Lee, Y.S. (Wonkwang University, Iksan, Republic of Korea) | Lee, J.H. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: johnhlee@chonbuk.ac.kr
We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([³H]-NE) into PC12 cells. The [³H]-NE incorporated into PC12 cells were then stimulated by a high concentration (60 mM) of K+ buffer during 12 minutes. Then, we collected 100 μl supernatant and counted the amount of [³H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS11052 from Streptomyces spp. which inhibited [³H]-NE release from PC12 cells.
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