Characteristics of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Production by Ralstonia eutropha NCIMB 11599 and ATCC 17699
2005
Song, J.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, B.S. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
Ralstonia eutropha NCIMB 11599 and ATCC 17699 were grown, and their productions of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] compared. In flask cultures of R. eutropha NCIMB 11599, cell concentration, P(3HB-co-4HB) concentration and polymer content decreased considerably with increases in the γ-butyrolactone concentration, and the 4HB fraction was also very low (maximum 1.74 mol%). In fed-batch cultures of R. eutropha NCIMB 11599, glucose and γ-butyrolactone were fed as the carbon sources, under a phosphate limitation strategy.
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