Improvement of Proliferation Capacity of Non-adapted CHO Cells Subcultured Using Serum Free Media in Long-term Culture
2006
Lee, S.S. (Konkuk University, Seoul, Republic of Korea) | Lee, J.S. (Konkuk University, Seoul, Republic of Korea) | Byun, S.H. (Konkuk University, Seoul, Republic of Korea) | Park, H.W. (Hanyang University, Seoul, Republic of Korea) | Cgiem, T.B. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
Animal cell culture industry has a large market and an exponential growth rate among biological industry field. Chines hamster ovary (CHO) cells are the most widely used cell lines for recombinant protein production. They can avoid infection from polio, herpes, hepatitis B, HIV, measles, adenovirus and etc. Moreover it is easy to transfection recombinant genes and possible to suspension culture. Serum free media is one of the most important factor of protein production. Because serum has problems.
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