Comparative Expression and Characterization of Dehydroascorbate Reductase cDNA from Transformed Sesame Hairy Roots using Real-Time RT-PCR
2006
Chun, J.A. (Donga University, Busan, Republic of Korea) | Seo, J.Y. (Donga University, Busan, Republic of Korea) | Han, M.O. (Donga University, Busan, Republic of Korea) | Lee, J.W. (Donga University, Busan, Republic of Korea) | Yi, Y.B. (Donga University, Busan, Republic of Korea) | Park, G.Y. (National Agricultural Products Quality Management Service, Busan, Republic of Korea) | Lee, S.W. (Jinju National University, Jinju, Republic of Korea) | Bae, S.C. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea) | Cho, K.J. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea) | Chung, C.H. (Donga University, Busan, Republic of Korea), E-mail: [email protected]
The differential transcription activity of dehydroascorbate reductase (DHAR) was scrutinized in the transformed hairy roots, leaves, stems, roots, and developing seeds of sesame (Sesamum indicum L.). Its relative levels of expression were compared via the threshold cycle (C∧T) method, using real-time RT-PCR. Ubiquitous expression of DHAR in all organs was confirmed with both real-time and conventional RT-PCR. With the former, DHAR transcript levels were, unexpectedly, 4.7-fold higher in the stem tissue than in the hairy roots; the lowest levels were detected in the seeds.
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