Effects of mutagenic sodium azide (NaN3) on in vitro development of four pea (Pisum sativum L.) cultivars

Turkan, A.D. . Dept. of Field Crops; Khawar, K.M.; Ciftci, C.Y.; Ozcan, S.

Effects of mutagenic sodium azide (NaN3) on in vitro development of four pea (Pisum sativum L.) cultivars

2006

Turkan, A.D. (Ankara Univ. (Turkey). Dept. of Field Crops) | Khawar, K.M. | Ciftci, C.Y. | Ozcan, S.

The study examined mutagenic effects of 0.001-0.005 M NaN3 with or without BAP or TDZ in MS medium on M1 generation of four pea cultivars Winner, Sprinter, Bolero and Karina. LD50 results showed that 0.001 M NaN3 was most appropriate for the creation of mutagenesis and was not highly lethal. Seeds treated with 0.001 M NaN3 grown on MS medium containing various concentrations of Benzylaminopurine (BAP) and Thidiazuron (TDZ) showed that BAP with NaN3 was better compared to TDZ with NaN3. Although, no effect of mutagen NaN3 was observed on rooting, presence of BAP or TDZ in the culture medium significantly affected this attribute. Shoots previously cultured on BAP with NaN3 rooted better on MS medium containing 2.5 muM NAA; compared to shoots previously cultured on MS medium containing TDZ with NaN3.

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