Introduction of new plum rootstock, Fereley Jaspi, in the propagation of certified planting material by the in vitro method of micropropagation
2007
Vujovic, T.,Institut za istrazivanja u poljoprivredi Srbija, Cacak (Serbia). Centar za vocarstvo i vinogradarstvo | Ruzic, Dj.,Institut za istrazivanja u poljoprivredi Srbija, Cacak (Serbia). Centar za vocarstvo i vinogradarstvo | Milenkovic, S.,Institut za istrazivanja u poljoprivredi Srbija, Cacak (Serbia). Centar za vocarstvo i vinogradarstvo
In 2005, introduction and propagation of basic or virus-free planting material of cultivars and rootstocks of pome, stone and berry fruit variaties was initiated at the Fruit Research Institute, Cacak (Serbia), within a project titled Introduction of Certification into Production of Fruit Planting Material. Micropropagation in vitro holds a prominent place in the system of certification, especially in the production of vegetative rootstocks, and Fereley Jaspi is mostly propagated by this method. Virus-free planting material originated from Germany (Lodder) was used as basic materia in this work. Each individual plant inderwent serological tests, to determine the possible presence of a virus. After the testing the plants were placed into a sterille substratum and kept in the screen-house conditions. In this way, the initial explants were taken straight from these plants. A standard-type procedure of surface sterilization was conducted, with the addition of time modifiucation. The aseptic culture was established on the medium Murashiga and Skoog (1962) (MS), mineral salts and organic complex with (in mg/l): BAP - 2.0, IBA - 0.5 and GA3 - 0.1/ The regenerated shoots stemming from each bud were treated as separate lines. After the rosette induction, during the multiplication phase, the shoots were also multiplied on the MS medium. mineral salts and organic complex with (in mg/l): BAP - 1.0; IBA - 0.1 and GA3 - 0.1. Th emonitored parameters of shoot multiplication were expressed through the multiplication index, as well as the length of the axial and the lateral shoots. The shoots that were rooted using the in vitro method were planted in a sterile soil substrate and successfully acclimatised in a greenhouse, using the mist system.
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