Antibacterial activity of crude extract of Punica granatum pericarp on pathogenic gram-negative bacilli.

Sirirak, T.. Faculty of Pharmaceutical Sciences. Dept. of Pharmacognosy and Pharmaceutical Botany; Supavita, T.. Faculty of Pharmaceutical Sciences. Dept. of Pharmacognosy and Pharmaceutical Botany; Panthong, K.. Faculty of Science. Department of  Microbiology; Voravuthikunchai, S.. Faculty of Science. Department of  Microbiology

Antibacterial activity of crude extract of Punica granatum pericarp on pathogenic gram-negative bacilli.

2005

Sirirak, T.(Prince of Songkla University. Hat Yai Campus, Song Khla (Thailand). Faculty of Pharmaceutical Sciences. Dept. of Pharmacognosy and Pharmaceutical Botany) | Supavita, T.(Prince of Songkla University. Hat Yai Campus, Song Khla (Thailand). Faculty of Pharmaceutical Sciences. Dept. of Pharmacognosy and Pharmaceutical Botany) | Panthong, K.(Prince of Songkla University. Hat Yai Campus, Song Khla (Thailand). Faculty of Science. Department of Microbiology) | Voravuthikunchai, S.(Prince of Songkla University. Hat Yai Campus, Song Khla (Thailand). Faculty of Science. Department of Microbiology)

Objective of this study was to investigate the effect of crude extracts of Punica granatum Linn. Pericarp with 3 different solvents against pathogenic Gram-negative bacilli. Ethanolic extracts showed the antibacterial activity against all strains tested including enterohaemorrhagic Escherichia coli 4 strains (E. Coli O157: H7, E. coli O26: H11, E. coli O111: Nm, E. coli O22), Pseudomonas aeruginosa, Shigella boydii and Salmonella London. Inhibition zones ranged from 10.02 to 19.15 mm. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ranged from 0.09 to 3.13 mg/ml and 3.13 to 25 mg/ml, respectively. Aqueous extract had low antibacterial activity while crude chloroform extracts had no effect on the growth of these strains. Ethyl acetate and n-butanol fractions of P. granatum pericarp demonstrated high activity with the best MIC and MBC values of 0.02 to 0.78 mg/ml and 0.19 to 6.25 mg/ml, respectively. As ethanolic extract of P. Granatum was very effective against these pathogenic bacteria, further investigation on this plant species may provide alternative, but bioactive, medicines for the treatment of Gram-negative bacterial infection.

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