The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.)
2008
Ruzic, Dj.V.,Fruit Research Inst., Cacak (Republic of Serbia) | Vujovic, T.I.,Fruit Research Inst., Cacak (Republic of Serbia)
With the aim of optimization of in vitro multiplication of sweet cherry cv. Lapins the effect of following cytokinins added to the Murashige and Skoog medium were studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) at concentrations of 1, 2, 5, 10 and 15 microM, combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0, 0.5, 2.5 and 5 microM. Multiplication index, length of axial and lateral shoots, fresh and dry shoot weight (callus, stem and leaves - S + L), colour, leaf and callus size, leaf roll, incidence of chlorosis or necrosis along with occurrence of rhizogenesis, i.e. roots unusual for this phase of micropropagation, were monitored. The highest multiplication index as well as length of axial and lateral shoots was obtained on media with BA. Very poor multiplication, with large sized shoots and big leaves, was achieved on media with 2iP, TDZ and KIN, whereas in many combinations with 2iP, and particularly in those with KIN, rhizogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of sweet cherry is limited to BA. For more rapid micropropagation, through joining rooting and multiplication phases, KIN and 2iP may be applied. The latter two may be also used to obtain sturdy shoots (elongation phase, prior to rooting).
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