Neurochemical characteristics of paracervical ganglion in the pig
2008
Podlasz, P.,University of Warmia and Mazury, Olsztyn (Poland). Faculty of Veterinary Medicine | Wasowicz, K.,University of Warmia and Mazury, Olsztyn (Poland). Faculty of Veterinary Medicine
A study on the presence of selected biologically active substances in nerve structures of the paracervical ganglion in the pig was performed with the use of immunofluorescence and RT-PCR. Immunohistochemical methods revealed that 23% of paracervical ganglion (PCG) neurons contain both tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DbetaH) and that the remaining 77% contain choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT); 73% of TH/DbetaH neurons contained neuropeptide Y (NPY) and 8% contained somatostatin (Som). All ChAT/VAChT positive neurons contained vasoactive intestinal polypeptide (VIP), 87% of them contained Som, 76% contained NPY and 32% contained neuronal nitric oxide synthase (nNOS). Galanin (Gal) was found only in small cells which were thought to be SIF cells. No pituitary adenylate cyclase-activating polypeptide (PACAP)- or substance P (SP)-positive neurons were found in PCG. Some areas of PCG contained dense plexuses of ChAT- and VAChT-positive nerve fibres. In the ganglion a small number of TH-, nNOS-, NPY-, VIP-, Gal-, PACAP-, Som- and SP-positive nerve fibres was also visible. RT-PCR detected the presence of mRNA for TH, ChAT, nNOS, NPY, VIP, Gal and Som which were visualised as clearly discernible bands on a gel. In cases of PACAP and SP only weak bands were observed.
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