QTL Analysis Of Morphological Traits In Arabidopsis Thaliana Using Recombinant Inbred Lines Under Water Stress Condition
2006
Farshadfar, E., Professor of Plant Breeding, College of Agriculture, Razi University | Farshadfar, F., Assistant Professor of Plant Breeding, Kermanshah Research Centre of Agriculture and Natural Resources
To locate QTLs controlling morphological characters of Arabidopsis thaliana under waterstress condition, 93 recombinant inbred lines resulted from the cross between two ecotypes of Colombia and Niederzenz were tested using factorial experiment in a randomized complete block design with five replications. The charsize of rosette, plant height, number of branches, number of silique and flowering time were measured during the experiment. The results of analysis of variance showed significant differences between ecotypes for all the characters measured indicating the presence of genetic variation. The results of QTL analysis using marker regression indicated that under water stress condition flowering time is controlled by 3 QTLs located on the chromosomes 1 and 4. Plant height is controlled by one QTL located on chromosome 1. Number of leaf and the size of rosett are controlled by two QTLs located on chromosome 1. Number of branch and silique are also controlled by one QTL located on chromosome 1. The contribution of QTLs controlling flowering time, plant heigh number of leaf, size of rosett, number of branch and number of silique in the total genetic variance was almost 90%, 72%, 89% and 47%, respectively.
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